Es la edad o etapa en la que un organismo se puede reproducir. A veces se considera sinónimo de edad adulta, aunque son dos conceptos distintos. En los seres humanos, el proceso de maduración sexual se denomina pubertad.
En organismos superiores, la madurez sexual es consecuencia de una maduración de los órganos reproductivos y de la producción de gametos. También puede ir acompañada de una fase acelerada de crecimiento u otros cambios físicos que distinguen al organismo inmaduro de su forma adulta. Los cambios físicos se denominan características sexuales secundarias, y, a menudo, representan un aumento en el dimorfismo sexual. Por ejemplo, antes de la pubertad, niños y niñas tienen el pecho plano, pero tras la pubertad se produce la diferencia al aparecer senos en las mujeres.
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